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Good's Buffer — MES

MES, the abbreviation for 2-(N-morpholino)ethanesulfonic acid, is a synthetic zwitterionic buffer. It was developed in 1966 by Norman Good and his team, belonging to the famous "Good's Buffers" family, and is one of the commonly used and important pH buffers in biochemical and molecular biology experiments.As a member of the "Good's Buffers" family, MES possesses a series of superior characteristics:① Ideal pKa value: The pKa of MES is 6.15 at 25°C. According to buffer theory, a buffer is most effective within ±1 pH unit of its pKa. Therefore, the ideal buffering range for MES is pH 5.5 - 6.7. This range covers the critical pH for many biochemical reactions.②Good biocompatibility: It causes minimal interference with enzyme activity and cell metabolism; has low cytotoxicity, making it commonly used in cell culture and protein studies.③Chemical inertness: Does not participate in or interfere with most biochemical reactions; does not strongly chelate essential divalent cations (such as Mg²?, Ca²?), which is crucial for the activity of many enzymes (e.g., ATP-related enzymes).④High water solubility: Highly soluble in water, allowing convenient preparation of high-concentration stock solutions (e.g., 1M).⑤Low UV absorption: Has very low absorbance at wavelengths above 260 nm, minimizing interference with UV spectrophotometric detection of nucleic acids or proteins.⑥Good thermal stability: Relatively stable at high temperatures.Given its superior physicochemical properties, MES offers significant advantages compared to other commonly used buffer components.MES vs. PBS (Phosphate Buffered Saline): PBS has strong buffering capacity in the pH 7.0-7.4 range but can chelate calcium and magnesium ions and may inhibit certain enzyme activities. MES does not have these issues and can work in more acidic pH conditions.MES vs. Tris: Tris has a pKa of 8.07 and is a classic buffer for the alkaline pH range. MES serves as a complement in the acidic to near-neutral range. Tris is temperature-sensitive and may participate as an active group in certain reactions.MES vs. HEPES: HEPES has a pKa of 7.55 and is one of the commonly used neutral buffers in cell culture. MES can serve as a complement to HEPES in more acidic pH ranges.Integrating the above advantages, MES finds wide application across various fields of life science research:? Protein Electrophoresis and Purification: SDS-PAGE / Chromatography.? Cell Biology: Used to prepare culture media or perfusion fluids to maintain pH stability in the extracellular environment.? Organelle Isolation: Provides a buffered environment in homogenization and centrifugation media for subcellular fractionation.? Biochemistry and Enzymology: Provides a stable pH environment for enzyme activity assays and enzyme kinetic studies.? Molecular Biology: Serves as a buffer component in Northern Blot and certain nucleic acid hybridization and detection experiments.In summary, MES is a high-performance, widely applicable buffer. Its core value lies in providing a stable, inert, and broadly compatible chemical environment within the weakly acidic physiological pH range of 5.5 - 6.7, ensuring the smooth progress of life science experiments.As a long-term, fixed supplier for many globally renowned culture media manufacturers and pharmaceutical companies, PanReac AppliChem provides reliable MES for researchers and pharmaceutical enterprises, leveraging its exceptional product quality and stringent quality control.ProductCodeChineseNameEnglishNameCASA06892-嗎啉乙磺酸MESanhydrous BioChemica4432-31-9 PanReac AppliChem is one of the high-quality manufacturers of biochemical reagents and pharmaceutical raw materials originating from Europe. With a century-long history, it has obtained multiple ISO quality management system certifications and serves as a key supplier to global life science research institutions, renowned CRO/CDMO companies, and large-scale industrial enterprises in the pharmaceutical, diagnostic, food, cosmetics, and other sectors.XMJ is the exclusive general distributor of PanReac AppliChem in China, providing users with comprehensive technical support and after-sales service. If you are interested in the products, please feel free to:· Call XMJ’s customer service hotline: 400-050-4006· Visit the official website: m.xh867.cn to learn more information. more>

New Product Recommendation | SISPROT? Proteomics Sample Preprocessing Kit

The sample preprocessing stage in proteomics experiments is a critical step to ensure the accuracy and consistency of experimental results. Given the complexity and numerous steps involved in the sample preprocessing workflow, developing an integrated and standardized experimental procedure is crucial for addressing the challenges in proteomics sample preprocessing. The SISPROT^®^ technology launched by BePepBio in 2023 is an integrated proteomics preprocessing technology based on the centrifugal pipette tip "Spintip". It enables one-stop completion of all steps in proteomics sample preprocessing from the crude sample, including protein sample pre-enrichment, reduction, alkylation, enzymatic digestion, and desalting. The processed samples can be directly used for LC-MS/MS analysis. This technology combines high sensitivity, high throughput, and high stability, making it particularly suitable for protein samples in the low microgram or even nanogram range.Product FeaturesHigh Efficiency: Complete sample processing within 2 hoursWhile traditional methods require 16 hours, the SISPROT Kit completes all sample preprocessing steps within 2 hours, and the processed samples are ready for direct instrumental analysis.High Sensitivity: Approximately 6000 proteins detected from 100ng Approximately 6000 proteins can be stably detected from 100ng. The minimum initial sample amount can be as low as 1ng (equivalent to ~10 cells).High Stability: Excellent quantitative reproducibility across different operatorsPopular ProductsSISPROT® SEStably identifies 7000+ proteins starting from just 500ng of protein, addressing the difficulty in detecting low-abundance proteins. Features simple operation and excellent reproducibility. Suitable for proteomics sample preprocessing of blood, body fluids, cells, exosomes, and other samples. Applicable in large-scale population cohort studies using blood samples and disease molecular subtyping research, high-throughput screening for drug targets, spatial proteomics, and other fields.SISPROT® Flex  Requires only 1-10μg of protein per operation for conventional samples, saving sample consumption. Compatible with SDS lysis buffer. Suitable for plant tissue, animal tissue, cells, plasma, serum, saliva, urine, cerebrospinal fluid, protein solutions, and other samples. Applicable in medical research fields such as biological mechanism exploration, diagnostic technology development, and therapeutic strategy research.Product InformationProduct NameCatalog No.SpecificationSISPROT® SE BO-008-9696T/KitBO-008-2424T/ KitBO-008-066T/ KitSISPROT® FLEX BO-012-2424T/ KitBO-012-066T/ KitRelated Literature ? Simple and integrated spintip-based technology applied for deep proteome profiling, Anal. Chem., 2016, 88(9):4864-71. ? 3D-SISPROT: A simple and integrated spintip-based protein digestion and three-dimensional peptide fractionation technology for deep proteome profiling, J. Chromatogr. A, 2017, 1498:207-214. ? Mixed-mode ion exchange-based integrated proteomics technology for fast and deep plasma proteome profiling, J. Chromatogr. A, 2018, 1564:76-84.Shenzhen Baymics Biotechnology Co., Ltd. is an R&D-focused high-tech company specializing in proteomics. Starting with its series of proteomics preprocessing products, the company aims to provide comprehensive workflow solutions for the target market through its clinical functional proteomics technology platform. The company is dedicated to reducing the technical complexity of proteomics analysis and enhancing its application throughout the entire chain of innovative biomarker and drug discovery, providing precise diagnosis and treatment strategies for major diseases using proteomics technology. As the exclusive China distributor for BePepBio, Beijing Ximeijie Technology Co., Ltd. has established long-term and stable partnerships with numerous well-known domestic pharmaceutical companies and CRO/CMO enterprises. Over the years, Ximeijie's products and services have helped many companies accelerate their R&D stages, improve drug quality, purity, and safety, optimize R&D processes, shorten time-to-market, and reduce QC costs. If you are interested in the aforementioned products, please call the Ximeijie customer service hotline at 400-050-4006 or visit the website m.xh867.cn for more information. more>

Mirus Universal Transfection Reagent: TransIT-X2? for Multiple Nucleic Acid/Protein and Cell Types

Based on research needs for delivering different types of nucleic acids/proteins, researchers often encounter the following three types of problems during cell transfection: · Cells are relatively sensitive to chemical reagents, leading to decreased cell viability or cell death after transfection; · A large variety of cell types are commonly used in research, and transfection efficiency varies significantly among different cell types. As a result, a single transfection reagent or experimental protocol cannot meet the experimental requirements; · Delivering multiple types of nucleic acids or proteins requires selecting corresponding transfection reagents and conducting extensive experimental optimization to achieve high transfection efficiency. Is there a chemical transfection reagent that can handle both conventional and hard-to-transfect cell types, has low cytotoxicity, and is suitable for delivering multiple types of nucleic acids/proteins? The TransIT-X2® Transfection Reagent, which combines multiple functions and high performance, can handle both common and hard-to-transfect cell types simultaneously. It eliminates the need for condition exploration and extensive repetitive experiments, allowing you to easily obtain your desired experimental results. · It has been verified to exhibit excellent transfection efficiency, low cytotoxicity (especially compared to Lipofectamine® series reagents that form liposome complexes), and high performance in most cell types (such as adherent/suspension cells, primary cells, and neural cells); · It is suitable for a wide range of research fields, including but not limited to stem cell research, CRISPR-based gene editing research, RNAi gene interference/silencing, stable cell line generation, low-toxicity transfection experiments, and co-transfection experiments; · It enables efficient and stable simultaneous delivery of multiple types of nucleic acids/proteins, including but not limited to plasmid DNA, siRNA/miRNA, and CRISPR/Cas9 components. Why does the Mirus TransIT-X2® Dynamic Delivery System possess such high performance and broad adaptability? This is attributed to Mirus’ powerful and efficient patented delivery platform design, which enables screening and optimization of transfection reagent components for multiple and multifunctional combinations. Founded in 1995, Mirus has focused on and specialized in the field of nucleic acid delivery for many years (Figure 1). From the earliest low-toxicity TransIT®-LT1 to the GMP-grade TransIT-VirusGEN® (which can be used for AAV and LV production), all products are designed and developed based on this delivery platform. To date, more than 10,000 research papers have been published using Mirus products, and these papers and Mirus’ database cover over 1,200 cell types. For more papers and data queries, please click here. Figure 1 Mirus Product Milestones Unlike traditional single-component-based transfection reagents (such as cationic polymers or cationic liposomes), Mirus combines its patented polymer and lipid technologies in multiple ways to further improve transfection efficiency for addressing different cell types or specific applications. Without forming liposomal complexes (i.e., liposomes, which usually have cytotoxicity), this multi-combination approach yields a variety of high-performance transfection solutions. These solutions overcome multiple obstacles in the cell delivery process, enabling more efficient transfection and lower cytotoxicity (Figure 2). Figure 2 Schematic Diagram of the Principle of Mirus Transfection Reagents Based on the design of Mirus' powerful and efficient patented delivery platform mentioned above, combined with its unique intelligent iterative design process— which involves multi-component, multi-combination screening, optimization, and subsequent validation—each component in the transfection technology is optimized (Figure 3). Mirus has launched a series of classic transfection reagents to meet diverse needs: 1. Broad-Spectrum, High-Performance, Low-Toxicity Transfection Reagent Family: TransIT-X2®, TransIT®-LT1, TransIT®-2020 2. Transfection Reagent Family for Specific Cell Types: TransIT®-293, TransIT®-BrCa, TransIT®-CHO, TransIT-HeLaMONSTER®, TransIT®-Insect, TransIT®-Jurkat, TransIT®-Keratinocyte 3. Transfection Reagent Family for Specific Applications: · Viral Production: VirusGEN® Transfection Reagents and Matching Kits, TransIT®-Lenti · Protein/Antibody Production: TransIT-PRO®, CHOgro® Expression System, CHOgro® High Yield Expression System 4. Transfection Reagent Family for Oligonucleotide Delivery: TransIT®-Oligo, TransIT-siQUEST®, TransIT-TKO® 5. mRNA and Long-Chain RNA Delivery: TransIT®-mRNA Figure 3 Schematic Diagram of Mirus' Unique Intelligent Iterative Design for Transfection Reagent Optimization Process Performance Data Presentation of Mirus TransIT-X2® Transfection Reagent Figure 4 TransIT-X2® Exhibits High Transfection Efficiency in Multiple Cell Types, Including Primary CellsThe Mirus TransIT-X2® Dynamic Delivery System was used to deliver and express EGFP plasmid DNA into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC), and normal human dermal fibroblasts (NHDF) respectively.The experiment was conducted in 96-well plates, with the TransIT-X2® reagent added at a volume of 0.2-0.4 µl and DNA added at 0.1 µg (using reagent:DNA ratios of 2:1, 3:1, or 4:1). At 48 hours post-transfection, transfection efficiency was evaluated through three repeated detections using a Guava® easyCyte? 5HT flow cytometer. Luciferase-encoding plasmid DNA was transfected into A549 (A) or MDCK (B) cells using TransIT-X2® (Mirus Bio), Lipofectamine®2000 (Thermo Fisher Scientific), or Lipofectamine®3000 (Thermo Fisher Scientific). At 24 hours post-transfection, transfection efficiency was evaluated by luciferase activity, and cytotoxicity was assessed by quantifying LDH released from damaged cells. Compared with cells treated with Lipofectamine®2000 or Lipofectamine®3000, TransIT-X2® exhibited higher fluorescence intensity and lower cytotoxicity at the optimal reagent:DNA ratio. Figure 5 Compared with Lipofectamine® series transfection reagents (which are single-component and form cationic liposomal complexes), TransIT-X2® shows higher transfection efficiency and lower cytotoxicity Experimental Tips: For more specific cell types and recommendations on reagent usage, please refer to the details in：Cell-type specific transfection protocol recommendations (PDF). Performance Data Presentation of Mirus TransIT-X2® in RNAi Experiments Functional research related to gene silencing plays a crucial role in molecular and cellular biology, and chemical transfection also plays an important part in this research field. Common natural RNA molecules involved in the RNAi pathway include: · Small interfering RNAs (siRNAs): Short double-stranded RNAs (20-25 bp) generated by the cleavage of double-stranded RNA (dsRNA); · MicroRNAs (miRNAs): A class of short, single-stranded RNAs (20-22 nt) produced after the processing of non-coding RNAs. Another method for inhibiting gene expression using RNAi is short hairpin RNA (shRNA). These short RNA sequences can be expressed via viral or non-viral vector methods. For more detailed information, please refer to Mirus  Applications | RNAi Gene Silencing。 Figure 6 Schematic Diagram of Different RNAi Pathways Figure 7 In gene silencing studies based on siRNA nucleic acid delivery, TransIT-X2® exhibits higher gene silencing efficiency compared to Lipofectamine®2000The TransIT-X2® Dynamic Delivery System and Lipofectamine®2000 transfection reagents were used to transfect siRNAs (targeting endogenous proteins—GAPDH and AHA1). For the control group, normal human dermal fibroblasts (NHDF) were used to deliver non-targeting siRNA. The amount of TransIT-X2® added was 4 µl, the amount of Lipofectamine®2000 added was 6 µl, and the amount of siRNA added was 25 nM for both reagents. qRT-PCR was performed to measure the mRNA levels of GAPDH or AHA1 relative to 18s rRNA. At 48 hours post-transfection, the mRNA levels were normalized to those of the non-targeting control group. The error represents the standard deviation from three replicate well experiments. Figure 8 In gene silencing studies based on miRNAs (miRNA Precursor and miRNA mimic), TransIT-X2® exhibits higher gene silencing efficiency compared to Lipofectamine®2000The TransIT-X2® Dynamic Delivery System and Lipofectamine®2000 transfection reagents were used to transfect Pre-miR? hsa-miR-1 miRNA Precursor or mirVana? miRNA mimic, miR-1 (both have been validated to reduce PTK9 mRNA expression levels). Pre-miR? negative control was used to assess the baseline level of mRNA expression. The amount of both TransIT-X2® and Lipofectamine®2000 reagents added was 3 µl, with 50 nM miRNA added. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure PTK9 mRNA expression levels relative to 18s rRNA, and the results were normalized against the negative control. Performance Data of Mirus TransIT-X2® in CRISPR Gene Editing Experiments The modified and optimized bacterial CRISPR/Cas9 system has been widely applied in gene editing of mammalian cells. There are two common key components in CRISPR-based gene editing experiments: Cas9 protein and guide RNA (gRNA). When Cas9 protein cleaves the target genomic DNA, it triggers two endogenous repair mechanisms, namely non-homologous end joining (NHEJ) and homology-directed repair (HDR), to address DNA breaks formed in cells. The NHEJ-based cellular repair pathway is an imprecise and error-prone repair mechanism, which can introduce indels (insertions or deletions) that lead to loss of gene function. In contrast, the HDR-based cellular repair pathway requires additional homologous DNA as a repair template to achieve "precision" repair, allowing the insertion of specific genes or long-fragment sequences into the target gene. According to researchers' different experimental needs, CRISPR gene editing can be set up in multiple experimental configurations. This means addressing the transfection or even co-transfection of different nucleic acid types, with examples as follows: 1. Plasmid pDNA Transfection The two key components of a CRISPR experiment—Cas9 protein and gRNA—can be designed to be co-expressed in a single plasmid DNA (A); alternatively, a two-plasmid system can be used, where one plasmid expresses Cas9 protein and the other expresses gRNA (B); when a Cas9 nickase is used, two gRNAs are required, which may necessitate a three-plasmid system for delivery experiments. 2. Co-transfection of Plasmid DNA and RNA Oligonucleotides In this case, different from the above experimental design, gRNA is delivered in the form of oligonucleotides, which means the delivery experiment requires simultaneous transfection of plasmid DNA and RNA oligonucleotides (A and B). 3. Co-transfection of mRNA and RNA Oligonucleotides To avoid off-target effects caused by genomic integration of plasmid DNA, mRNA encoding Cas9 protein and gRNA (RNA oligonucleotides) can be co-transfected. 3. Delivery of Cas9/gRNA RNP Complexes As the commercialization of Cas9 protein and gRNA becomes increasingly mature, more and more researchers choose to directly purchase high-fidelity Cas9 protein and gRNA oligonucleotides with additional chemical modifications (which enhance their stability in cells) from third-party suppliers. Apart from greatly shortening and simplifying the CRISPR experimental workflow, the direct delivery of RNP complexes offers higher specificity and editing efficiency compared to the approaches that rely on plasmid- or mRNA-based synthesis of Cas9 and gRNA. Experimental Tips: For the specific experimental instructions optimized for the TransIT-X2® Dynamic Delivery System (tailored to the different CRISPR experimental designs and delivery scenarios mentioned above), the download link is as follows: TransIT-X2® for CRISPR Plasmid + gRNA Delivery (PDF) TransIT-X2® for CRISPR Plasmid Delivery (PDF) TransIT-X2® for CRISPR RNP + ssODN Delivery (PDF) TransIT-X2® for CRISPR RNP Delivery (PDF) The TransIT-X2® Dynamic Delivery System (2 µl/well in 24-well plates, Mirus Bio) was used to co-transfect 0.5 µg of plasmid DNA (expressing Cas9 protein) and 50 nM of 2-part gRNA (targeting the PPIB gene) into HEK293T/17, U2OS, and NHDF cells. At 48 hours post-transfection, T7E1 assay was performed to verify the cleavage efficiency. Figure 9 Co-transfection of Plasmid DNA and gRNA Oligonucleotides: TransIT-X2® achieved high gene editing efficiency (18-48%) when co-transfecting Cas9 plasmid DNA and gRNA (RNA oligonucleotides) into 293T/17, U2OS, and NHDF cells, respectively The 2-part gRNA (targeting PPIB gene, IDT) and Cas9 protein (PNA Bio) formed RNP complexes, which were respectively delivered into 293T/17 and U2OS cells using TransIT-X2® Dynamic Delivery System (1 µl/well, Mirus Bio), Lipofectamine® CRISPRMAX? (1.5 µl/well, ThermoFisher), Lipofectamine® RNAiMAX (1.5 µl/well, ThermoFisher), and Lipofectamine® 3000 (1.5 µl/well, ThermoFisher). Two usage amounts of gRNA were tested (6 nM or 12 nM) with the same amount of Cas9 protein added (6 nM). At 48 hours post-transfection, the T7E1 assay was performed to verify the cleavage efficiency. Figure 10 Delivery of Cas9/gRNA RNP Complexes: TransIT-X2® exhibits higher cleavage efficiency compared to Lipofectamine® series transfection reagents Advantages of Mirus TransIT-X2® Product ? A new broad-spectrum transfection reagent developed based on multiple components (suitable for various cells, including hard-to-transfect cells); ? Capable of co-transfecting nucleic acids and proteins, including but not limited to DNA, siRNA/miRNA, and CRISPR/Cas9 complexes; ? Does not form liposomal complexes, reducing cytotoxicity; ? Meets multiple applications: gene overexpression, gene silencing, gene editing, stem cell research, stable cell line construction, etc.Related Product Information Product Name SpecificationItem Number TransIT-X2® Dynamic Delivery System 1 × 0.3 ml MIR 6003 1 × 0.75 ml MIR 6004 1 × 1.5 ml MIR 6000 5 × 1.5 ml MIR 6005 10 × 1.5 ml MIR 6006 End-of-Article Benefit: Follow Ximeijie's official WeChat public account "xmjsci", reply with the keyword "X2 Trial", and fill in the information to have the opportunity to receive a free 100μl trial sample of TransIT-X2® Transfection Reagent. After a successful trial, you can also enjoy a discount when purchasing the full-size TransIT-X2®! Come and apply for the trial and make your purchase now! Founded in 1995, Mirus (USA) is one of the earliest companies in the world to develop high-efficiency and low-toxicity transfection reagents, and also one of the leading suppliers of such reagents currently. Recognized as a pioneer in transfection reagent development, Mirus has achieved numerous outstanding results that have gained global recognition and high praise from a wide range of users.Beijing XMJ Technology Co., Ltd. is the authorized distributor of Mirus in China. Adhering to a professional and rigorous attitude, we have been committed to providing customers with high-quality products and services. If you are interested in the aforementioned products, please feel free to send email to info@xmjsci.com or visit the official website m.xh867.cn to obtain more product information. more>

Cygnus CHO 6xLipase? MS Assay——Accurately Quantifying Risks, Clarifying Process Optimization Directions

In biopharmaceutical production, host cell protein (HCP) residuals are one of the key factors affecting drug stability. Among them, lipase-class HCPs are a major focus within HCP residuals due to their potential to degrade surfactants commonly used in formulations (such as Tween-80/20). Tween degradation leads to an increase in free fatty acids in the drug solution, which can induce protein aggregation, formation of fatty acid-protein complex particles, and subsequently impact product safety and efficacy. While the ELISA HCP detection method can assess overall HCP levels, it cannot identify specific types of lipases, making it difficult to precisely evaluate process-related risks. To address this issue, Cygnus Technologies has developed a targeted proteomics analysis method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) – the CHO 6xLipase? MS Assay. This method utilizes Parallel Reaction Monitoring (PRM) technology and Stable Isotope-Labeled peptides (SIL) to achieve absolute quantification of six CHO-derived HCP lipases known to cause Tween degradation:： HCP LOD (ppm) Quantification Range (ppm) Lysosomal Acid Lipase（LAL） 0.15 18.25 - 292.06 Lipoprotein Lipase（LPL） 0.08 0.10 - 323.34 Phospholipase A1（PLA1） 0.12 0.49 - 311.67 Group XV Lysosomal Phospholipase（GXVPA2） 0.03 4.72 - 302.31 Phospholipase B-Like 2（PLBL2） 0.56 0.66 - 419.46 Phosphoinositide Phospholipase C（PIPLC） 0.09 13.91 - 890.07 Technical RoadmapTechnical Advantages: ? Pharmacopeial Method: PRM combined with SIL peptides is an absolute quantification method recognized by the USP <1132.1> guideline, with accuracy and precision far exceeding semi-quantitative methods. ? Excellent Linear Range: All 6 lipases show R² ≥ 0.98, with a quantification range covering 0.1 - 890 ppm, meeting detection needs from early process stages to final product. ? Stringent Validation Standards: Accuracy error <35%, precision %CV <25%, Limit of Detection (LOD) as low as 0.03 - 0.56 ppm. Case Studies Client 1: High-Risk Sample (DS-top): Fc-Fusion Protein ? Tween20 degradation (complete degradation within 3 months) ? 5 lipases detected, multiple enzymes synergistically accelerating Tween degradation HCP Predicted Concentration (ng/mL) %CV GXVP_A2 1.15 5.2% LAL 2.76 9.6% LPL 6.94 2.3% PIPL_C NA PL_A1 0.23 3,2% PLBL2 12.4 Client 2: Stable Sample (DS-bottom): Monoclonal Antibody ? Tween20 degradation detected ? Only PLBL2 detected (3.06 ng/mL), the other 5 were below the limit of detection HCP Predicted Concentration (ng/mL) %CV GXVP_A2 NA LAL NA LPL NA PIPL_C NA PL_A1 NA PLBL2 3.06 3.20% Service Package: Standard Service Cycle: Approximately 6 weeks (from sample receipt to report issuance) Expedited Service: 4-week or 2-week options available (additional fees apply) Sample Requirement: 1 mL of DS sample quantified by BCA assay (concentration ≥ 4 mg/ml) If you are interested in our lipase residual detection service, please click here to fill out the form. XMJ will contact you promptly to share more detailed information. Cygnus Technologies, LLC. provides products and analytical methods for the biotechnology and biopharmaceutical industry, aiming to accelerate R&D stages and improve product quality. Cygnus develops and manufactures bioprocess residual kits for detecting specific impurities across over 50 different expression systems. As an expert in highly sensitive analytical techniques for biotechnology applications, particularly immunoassays, Cygnus's products and services have been used by nearly all major biopharmaceutical companies for over 25 years. Beijing XMJ Science & Technology Co., Ltd., as the exclusive distributor of Cygnus in China, has established long-term and stable cooperative relationships with numerous renowned domestic pharmaceutical companies and CRO/CMO enterprises. Over the years, XMJ's products and services have helped many companies accelerate R&D stages, improve drug quality, purity, and safety, optimize R&D processes faster, reduce time-to-market, and lower QC costs. If you are interested in the above products, please feel free to contact XMJ customer service hotline at 400-050-4006 or visit the website m.xh867.cn for more information. more>

【Strategic Cooperation】XMJ Joins Hands with Synthego to Bring Advanced CRISPR Technology Solutions

CRISPR, an acronym for "Clustered Regularly Interspaced Short Palindromic Repeats," refers to an adaptive immune mechanism found in bacteria and archaea. This system comprises Cas nucleases and guide RNAs (gRNAs) and was initially utilized by microorganisms to recognize and cleave viral DNA, thereby providing immunity against viral infections. It has since been engineered into a powerful gene-editing tool. Following the seminal discovery by Drs. Doudna and Charpentier in 2012 of the molecular mechanism underlying DNA cleavage mediated by the CRISPR-Cas9 system, extensive research and optimization efforts by the global scientific community have advanced this technology into a highly precise and broadly applicable genomic editing platform. Currently, CRISPR-based technologies are widely employed across diverse fields, including gene editing, RNA editing, base editing, molecular diagnostics, and live-cell imaging. They have enabled genetic modifications in a wide range of organisms, including humans, thereby accelerating advancements in both basic research and clinical applications. To achieve precise gene editing using CRISPR technology, the design of guide RNA (gRNA) and the selection of Cas nucleases are two critical factors. The choice of gRNA depends primarily on the specific type of genetic modification required, such as gene knockout (KO), gene knock-in (KI), base editing, prime editing, CRISPR interference (CRISPRi), or CRISPR activation (CRISPRa). Synthego offers a freely accessible CRISPR design tool available to researchers worldwide, which facilitates the design and selection of optimal gRNA sequences for targeted editing of over 120,000 genes. Furthermore, the appropriate selection of Cas enzymes is essential for experimental success. Cas9, originally derived from Streptococcus pyogenes (SpCas9), was the first widely adopted nuclease and remains the most commonly used. However, a growing range of natural and engineered Cas variants are now available for specialized applications. For example, Cas9 nickase induces single-strand breaks to enhance editing precision, while high-fidelity Cas9 variants significantly reduce off-target effects. Additionally, Cas13, a naturally occurring nuclease, demonstrates considerable potential in molecular diagnostics. In advancing the application and development of CRISPR technology, Synthego serves not only as a leading provider of integrated CRISPR solutions but also as a strategic partner committed to innovation and customer success. The company supplies all key components required for CRISPR-based gene editing, including gRNA design and synthesis services, as well as a comprehensive portfolio of natural and engineered gene editing enzymes. Its product offerings span research-grade, clinical-grade, and GMP-grade materials, supporting researchers throughout the entire pipeline—from basic research to clinical translation. Research-grade product for preclinical research and development Within Synthego's product portfolio, research-grade products hold a central position, specifically developed to support early-stage preclinical research. Notably, these products are manufactured under the same rigorous quality control standards as GMP-grade materials, ensuring high reliability and reproducibility of experimental outcomes. Synthego's research-grade gRNAs have been widely adopted by leading academic institutions, biotechnology firms, and pharmaceutical R&D organizations globally, and are recognized as an industry benchmark in CRISPR-based reagent synthesis. This positions Synthego as one of the few companies capable of delivering research tools that meet clinical-level standards of rigor. The INDe solution helps optimize the early clinical stage Establishing a robust bridge between early preclinical research and regulatory compliance remains one of the most significant challenges in the development of CRISPR-based therapies. This transitional phase often acts as a critical bottleneck, potentially resulting in project delays or developmental stagnation. Synthego's INDe solution is specifically designed to address this pivotal stage by providing cost-effective, moderately scalable, quality-controlled, and customizable options tailored to the demands of preclinical optimization. Synthego's INDe gRNAs enable researchers to validate candidate therapeutics, refine editing processes, and establish a solid foundation for clinical-scale manufacturing. Synthego INDe solutions "GMP-like" processes INDe solutions are invaluable for validating therapeutic targets under conditions that closely mimic GMP standards. INDe gRNAs include documentation like batch records, traceability, and validation to support your IND submissions and early-phase trials, while helping you avoid the expense of full GMP until you truly need it, so you can maximize your budget across development milestones. By using INDe, you gain insights that will smooth the transition into GMP-grade production, cutting down time and costs associated with re-validation. The reliance on "GMP-like" processes has become widespread for producing materials intended for IND-enabling studies and clinical use. Yet, "GMP-like" processes often lack the documentation, quality oversight, and comprehensive controls required to meet the demands of regulatory submissions. They frequently result in variability and insufficient traceability, which can delay regulatory approvals, and hinder the safety and efficacy of therapeutic products. GMP-Grade Products for Clinical Trials Synthego’s GMP-grade gRNAs and nucleases set the benchmark for quality, reliability, and compliance in CRISPR clinical manufacturing. Developed under strict Good Manufacturing Practice (GMP) guidelines, these products are tailor-made to meet the precise standards required for IND submissions and therapeutic-grade applications. With Synthego as your partner, you’ll benefit from cutting-edge manufacturing capabilities coupled with the assurance of meeting even the most demanding regulatory requirements. Having supported over 12 IND submissions for CRISPR therapies, Synthego has become a trusted name in the industry. Leaders continue to rely on us not just for our products, but for the assurance that their work is built on a foundation of excellence. Our GMP offering stands as a testament to our commitment to revolutionize the field of gene editing with therapeutic outcomes in mind. Synthego is a pioneering force in the biotechnology industry, dedicated to advancing the frontiers of CRISPR cell and gene therapies through cutting-edge CRISPR technology and expertise. Our mission is to provide unparalleled access to best-in-class CRISPR solutions at scale, empowering agility in life science research and development from discovery through clinical trials. From streamlined approaches to licensing through full technical and regulatory support from our industry-leading experts, Synthego is enabling greater access to CRISPR technology driving to improved outcomes in patient therapeutic treatments. With its technologies cited in hundreds of peer-reviewed publications and utilized by thousands of commercial and academic researchers and therapeutic drug developers, Synthego is at the forefront of innovation, enabling the next generation of medicines by delivering genome editing at an unprecedented scale. XMJ Scientific is the distributor of Synthego in China, providing users with comprehensive technical support and after-sales service. Welcome to call XMJ's customer service hotline at 400-050-4006 or visit the website m.xh867.cn for more information at any time. more>

Recommendation | CRISPR / Cas Systems

CRISPR and Cas proteins collectively constitute the adaptive immune system in prokaryotes and have been harnessed as highly precise molecular tools for targeted editing of DNA and RNA. The Cas9 protein (CRISPR-associated protein 9), in particular, has emerged as the cornerstone of genome editing due to its capacity to generate site-specific double-strand breaks in genomic DNA, enabling programmable and efficient genome modification. In various model organisms, these double-strand breaks are predominantly repaired through the non-homologous end joining (NHEJ) pathway, often resulting in insertions or deletions that lead to gene inactivation; alternatively, when a repair template is provided, the homologous recombination (HR) pathway facilitates precise integration of exogenous genetic sequences. In human cells, the CRISPR/Cas9 system has been rigorously validated for applications in gene knockout and gene replacement, demonstrating robust efficacy in correcting disease-causing mutations and offering a transformative approach for the treatment of monogenic disorders. As gene editing technologies continue to advance in precision, efficiency, and safety, and as their applications expand across diverse fields, Cas9-based systems exhibit substantial potential in gene therapy, agricultural biotechnology, and the development of novel biopharmaceuticals, positioning them as pivotal instruments in advancing both fundamental research and translational life sciences. CRISPR/Cas9 DNA Editing To support Cas9-related research，Cell Biolabs, distributed by XMJ, offers two types of Cas9 detection kits.：Our Cas9 ELISA Kits provide a convenient way to quantify Cas proteins in a 96-well plate format. Our Cas9 Activity Assay Kits are enzymatic kits that measure Cas9 activity levels in cell lysates. Both formats are available to test Cas9 from either Staphylococcus aureus or Streptococcus pyogenes. Cas9 Activity Assay KitsCell Biolabs’ Cas9 Activity Assay Kit is an enzymatic assay developed to test the activity of Cas9 protein in cell lysate samples.Cat No.Product NameSizeSensitivityPRB-5203SaCas9 Activity Assay Kit (S. aureus)20 assays4 µg/mLPRB-5206SpCas9 Activity Assay Kit (S. pyogenes)20 assays10 µg/mL Cas9 ELISA KitsCell Biolabs’ Cas9 ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of Cas9 in cell or tissue lysate samples.Cat No.Product NameSizeSensitivityPRB-5204SaCas9 ELISA Kit (S. aureus) 96 assays3.9 ng/mLPRB-5079SpCas9 ELISA Kit (S. pyogenes)96 assays1.5 ng/mL Cell Biolabs, Inc. proudly develops and commercializes innovative technologies and tools for life science research. We are committed to providing the best products that facilitate discovery of the mechanisms underlying cell function and disease. Our unique, cutting-edge products are currently used around the world in the research laboratories of universities, government institutes, biotechnology and pharmaceutical companies. XMJ Scientific is the distributor of Cell Biolabs in China, providing users with comprehensive technical support and after-sales service. Welcome to call XMJ's customer service hotline at 400-050-4006 or visit the website m.xh867.cn for more information at any time. more>

SeraCare?KPL Immunological Support Reagents

The LGC·SeraCare·KPL (hereinafter referred to as KPL) that XMJ represents is a globally renowned commercial secondary antibody and substrate coloration system producer with a product development experience of over 40 years. It has obtained ISO 13485 quality system certification and its products are stable batch-to-batch with reliable quality, which is highly favored and praised by numerous diagnostic enterprises. The product line of KPL includes cost-effective secondary antibodies, substrate coloration reagents, and immunological support reagents, and is widely used in immunological research, diagnostic reagent development, and clinical testing fields. This article will focus on introducing the immunological support reagent products of KPL. Immunological testing has extremely important applications in various fields such as medicine and biological research, including disease diagnosis, vaccine development, and drug screening. Accurate and reliable test results are of vital importance for scientific research and clinical practice. Immunoassay support reagents play an indispensable role in the immunological testing process, functioning like the lubricant and stabilizer of a precision instrument, ensuring the smooth, efficient and accurate operation of the entire testing process. Buffer solutions play a crucial role in maintaining stability in immunological tests. They ensure an appropriate pH range, facilitate the smooth conduct of biochemical processes such as antigen-antibody reactions in a stable environment, and prevent deviations in test results due to pH fluctuations. The KPL brand buffer solutions, with their unique formulation, guarantee the stability of the buffer solutions and are compatible with various testing systems and reagents. Product Name MaterialNumber Size Description 10X Phosphate Buffered Saline (PBS) 5460-0022 5460-0023 1 Bottle x 1.0 L 1 Bottle x 5 L 10 times concentrated solution, suitable for mostimmunological experiments. 10X Phosphate Buffered Saline with Tween 20(PBST) 5460-0026 5460-0027 1 Bottle x 200 mL 1 Bottle x 1.0 L 10 times concentrated solution, Tween 20 can reduce non-specific binding andprotein-protein interactions. 10X Dulbecco's PBS with Tween 20 (D-PBST) 5460-0033 5460-0034 1 Bottle x 200 mL 1 Bottle x 1.0 L 10 times concentrated solution, improvedformula, without magnesium salts or calcium salts in the buffer solution. The blocking solution plays a crucial role in immunological testing by pre-binding to the blank sites in the detection system (such as the blank protein binding sites in ELISA plate wells), preventing the adsorption of non-specific proteins and reducing false positive results caused by non-specificity, thereby ensuring the accuracy of the results. Common components of blocking solutions include non-protein-based blockers and protein-based blockers. The KPL brand blocker has high stability and is not easily affected by changes in experimental conditions such as temperature and pH value. Even in complex test samples or high-concentration protein environments, it can effectively prevent non-specific binding and significantly improve the accuracy of the test.Product Name MaterialNumber Size Description 10% BSA Diluent/ Blocking Solution 5140-0006 5140-0008 2 Bottles x 100 mL 1 Bottle x 1.0 L 10% BSA blocking solution/diluent, avoidingextensive cross-reactions, compatible with most detection systems, suitablefor applications such as ELISA, WB, IHC, etc. Milk Diluent/Blocking Concentrate Kit 5140-0011 2 Bottles x 100 mL 2% skimmed milk blocking solution/diluentsignificantly reduces non-specific results and is suitable for applicationssuch as ELISA and WB. The washing solution is used to remove unbound antigens, antibodies, non-specifically bound proteins and other impurities, reducing background interference and enhancing the contrast between the detection signal and the background, thereby improving the sensitivity and specificity of the detection,during the immunological detection process. The surfactants and salts in the washing solution work synergistically to achieve an effective washing effect, while avoiding damage to the target detection substances. The unique formula of KPL washing agent enables efficient removal of impurities under mild conditions, significantly reducing the background noise in the detection results. KPL washing solution is available in concentrated form, allowing for rapid preparation of the desired concentration according to experimental requirements.Product Name MaterialNumber Size Description Wash Solution Concentrate Kit(20X) 5150-0008 5150-0011 4 Bottles x 200 mL 1 Bottle x 1.0 L 20 times concentrated solution, containingimidazole buffer and Tween 20, is suitable for applications such as ELISA, WBand IHC. The stop solution terminates the enzymatic reaction during the immunological detection process by altering the reaction conditions (such as changing the pH value), ensuring that the generation of reaction products is interrupted at a specific moment, so as to accurately measure the amount of reactants and thereby calculate the concentration of the analyte. The KPL termination solution can quickly terminate the reaction in a short period of time and maintain a stable state, thus ensuring the reliability of the detection results.Product Name MaterialNumber Size Description ABTS&reg; Peroxidase Stop Solution 5150-0017 2 Bottles x 100 mL Patented formula, applicable to the ABTSsubstrate system. TMB BlueSTOPTM Solution 5150-0022 5150-0024 4 Bottles x 100 mL 1 Bottle x 1.0 L Ready-to-use patented formula, suitable forTMB substrate system. Different from traditional acid termination solution,it maintains a stable blue color even after termination. APStop? Solution (10X) 5150-0026 2 Bottles x 100 mL Patented formula, applicable to alkalinephosphatase and pNPP detection systems. Stabilizers are used to maintain the stability and activity of enzyme markers (such as horseradish peroxidase HRP or alkaline phosphatase AP), thereby ensuring the accuracy and reproducibility of experimental results. Commonly used stabilizers include glycerol, BSA, diethanolamine, and other commercial products. KPL stabilizers are ready-to-use solutions and are widely used in applications that require enzyme markers, such as ELISA, Western blot (WB), and immunohistochemistry (IHC).Product Name MaterialNumber Size Description HRP Stabilizer 5290-0005 1 Bottles x 200 mL HRP stabilizer, used for diluting andpreserving HRP markers. It has better preservation effect and stabilitycompared to BSA and 50% glycerol, and can be used directly. It has a longerpreservation time. AP Stabilizer 5290-0007 1 Bottles x 200 mL AP stabilizer, used for diluting andpreserving AP markers. It has better preservation effect and stabilitycompared to BSA and 50% glycerol, and can be used for a longer period oftime. It is an ready-to-use solution. An enhancer is a reagent used to increase the detection sensitivity and signal intensity. During the WB experiment, it can enhance the antibody signal that binds to the target protein on the membrane, which helps to improve the signal-to-noise ratio of low-quality and low-affinity antibodies, reduce background noise, and make the WB results clearer.Product Name MaterialNumber Size Description TMB Membrane Enhancer 5420-0026 1 Bottles x 40 mL To enhance the signal effect, it isrecommended to use in conjunction with the two-component TMB substrate (itemnumber: 5120-0047). Fluorescence mounting media is commonly used in immunological experiments such as immunofluorescence (IF), fluorescence in situ hybridization (FISH), and flow cytometry, to protect samples from external environmental interference, reduce photobleaching, and enhance signal stability. KPL's fluorescent mounting media is a glycerol-based solution based on immunofluorescence microscopes. Its unique formulation contains inhibitors that can significantly slow down the photobleaching process of fluorescent markers such as fluorescein and rhodamine, and can also be used as a diluent for fluorescently labeled conjugates.Product Name Material Number Size Description Fluorescent Mounting Media 5570-0005 1 Bottles x 15 mL Recommended for use in immunohistochemistry andfluorescence in situ hybridization experiments, and can be used for thepreparation of various cell slides. KPL (Kinetic Plasmonics Laboratories) is a company with a profound history and an outstanding reputation in the field of biological sciences. As one of the world’s early biotech companies to commercialize affinity-purified secondary antibodies, KPL has laid a solid foundation for its development by virtue of its pioneering position in this field. In addition, KPL is also one of the world’s leading manufacturers of secondary antibodies and chromogenic substrate systems, boasting nearly 40 years of experience in product R&D, as well as a wealth of professional knowledge and technical capabilities accumulated over the years. The company has obtained ISO 13485 quality management system certification, which ensures that all its products have minimal batch-to-batch variation and reliable quality. Characterized by high purity, high sensitivity, and an excellent signal-to-noise ratio (strong signal with low background), KPL’s products are highly trusted by researchers and diagnostic enterprises. In 2013, KPL was acquired by SeraCare, a supplier of in vitro diagnostic (IVD) reagents, further consolidating its leading position in the industry. Founded in 1984, SeraCare is a major partner for global IVD manufacturers and clinical laboratories, and became part of the LGC Group in 2018. As the exclusive distributor of the LGC·SeraCare·KPL brand in China, Beijing XMJ Biotechnology Co., Ltd. is committed to promoting KPL’s high-quality products in the Chinese market and providing domestic researchers with premium products and professional services. XMJ is the agent of LGC·Seracare·KPL brands in China. dedicated to promoting the excellent products of KPL in China and providing high-quality products and professional services to the vast number of scientific researchers in the country. If you are interested in the products, please feel free to call the national customer service hotline of XMJ at 400-050-4006 or visit the official website: m.xh867.cn to learn more information. more>

SeraCare-KPL Cost-Effective wB Substrate

LGC·SeraCare·KPL (hereinafter referred to as KPL), distributed by Ximeijie, is an internationally renowned manufacturer of commercially available affinity-purified secondary antibodies and substrate chromogenic systems. It has over 40 years of experience in product R&D and production, and has obtained ISO 13485 quality management system certification. Its products feature high batch-to-batch consistency and stable, reliable quality, which have won wide recognition and trust in the diagnostic reagent industry. KPL's product portfolio includes cost-effective secondary antibodies, substrate chromogenic reagents, and immunoassistive reagents, etc., which are widely used in fields such as immunological research, diagnostic reagent development, and clinical testing. KPL's cost-effective substrates and general-purpose reagents can be extensively applied to various experimental platforms, including Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot (WB), Immunohistochemistry (IHC), and Enzyme-Linked Immunospot Assay (ELISpot). This article will focus on introducing the cost-effective substrate product series under the KPL brand that are suitable for Western blotting technology. Western Blot technology Western Blot technology is an immunoblotting technique used for detecting specific proteins, and it is one of the most popular methods in the field of immunoassays. Based on protein separation by molecular weight and specific antibody recognition, it involves separating proteins via electrophoresis, transferring them onto a solid-phase membrane, and then using specific antibodies for detection. In a Western Blot experiment, signal detection is a critical step, serving to visualize and quantify the target protein. In the past, colorimetric detection and low-sensitivity chemiluminescent detection drove the expansion of related research. However, with the continuous increase in requirements for detection sensitivity and advancements in imaging analysis systems, the design of chemiluminescent molecules has also achieved significant progress, enabling detection with higher sensitivity. In the Western Blot experimental system, high-quality substrates are crucial for achieving high sensitivity and low background signals. The chromogenic substrates and chemiluminescent substrates provided by KPL, with their excellent performance and reliable quality, have become core components of the Western Blot experimental system, helping researchers obtain more ideal experimental results. Chemiluminescence Chemiluminescence is the optimal choice for detecting trace proteins. Its principle lies in the luminescence of a chemiluminescent substrate when catalyzed by an enzyme (such as HRP or AP) labeled on an antibody. KPL’s LumiGLO® series consists of luminol-based HRP substrates, offering a complete range of detection solutions from regular to ultra-high sensitivity. KPL’s PhosphaGLO? series, on the other hand, are dioxetane-based AP substrates that combine high sensitivity with operational flexibility. Both series are suitable for Western Blot and ELISA applications. LumiGLO Reserve? & LumiGLO® Chemiluminescent Substrates Both LumiGLO Reserve? and LumiGLO® are luminol-based chemiluminescent substrates, specifically designed for high-sensitivity detection. LumiGLO Reserve?: Its sensitivity is over 20 times higher than that of LumiGLO® and other similar products, making it particularly suitable for detecting low-abundance proteins or analyzing precious samples. The luminescent signal can last for 4 to 8 hours, with the strongest signal appearing within the first hour after the reaction. The luminescence intensity is significant, allowing for easy and clear capture by chemiluminescent imagers. This product is supplied as a stable two-component solution, which can be prepared for immediate use at a 1:2 ratio. It is available in three specifications, along with matching concentrated KPL wash buffers, further enhancing experimental convenience. LumiGLO®: Also provided as a two-component solution, it can be used simply by mixing equal volumes of the two components, ensuring easy operation. In Western Blot and ELISA detection, it is more sensitive than chromogenic substrates and produces lower background. The maximum luminescence intensity is achieved within 5 minutes after reaction with HRP, and the signal can persist for 1 to 2 hours. It supports multiple stripping and re-probing of immunoblot membranes, and its linear dynamic range is superior to that of most similar products. Thus, it is widely applicable to various protein detection experiments. The detection results of both products can be permanently recorded using X-ray films or chemiluminescent imagers. Product Name Item Number Specification Reactive Enzyme / Catalytic Enzyme Signal Type Sensitivity LumiGLO Reserve? Chemiluminescent Substrate 5430-0050 5430-0051 5430-0049 600 cm2 1000 cm2 2400 cm2 HRP Chemiluminescence 10-15-10-12 LumiGLO® Chemiluminescent Substrate 5430-0042 5430-0040 5430-0041 60 mL 240 mL 720 mL HRP Chemiluminescence 10-12 PhosphaGLO Reserve? AP & PhosphaGLO? Chemiluminescent Substrates Both PhosphaGLO Reserve? and PhosphaGLO? are dioxetane-based, ready-to-use, single-component chemiluminescent substrates, specifically optimized for alkaline phosphatase (AP)-labeled systems. PhosphaGLO Reserve?: It features ultra-high sensitivity at the femtogram level, enabling accurate detection of low-abundance proteins while significantly reducing the consumption of precious samples. It provides excellent signal intensity and stable luminescence for up to five days, supporting multiple exposures or repeated readings. Its signal intensity is significantly superior to that of traditional chromogenic substrates and other chemiluminescent products. PhosphaGLO?: It enables stable detection of proteins at the picogram level, requiring no additional blocking steps for simple operation. The luminescent signal can persist for up to five days, and the product can be stably stored at 4°C for two years. Both products are compatible with various detection devices such as X-ray films and chemiluminescent imagers, providing a flexible and reliable solution for high-sensitivity protein detection. Product Name Item Number Specification Reactive Enzyme / Catalytic Enzyme Signal Type Sensitivity PhosphaGLO Reserve? AP Substrate 5430-0052 5430-0053 30 mL 100 mL AP 化學(xué)發(fā)光 10-15 PhosphaGLO? AP Substrate 5430-0054 5430-0055 30 mL 100 mL AP 化學(xué)發(fā)光 10-12 Chromogenic Substrate Product Series Chromogenic substrates utilize enzymatic reactions to generate visible end products, making them suitable for detection scenarios where the target protein content is relatively high. The advantage of such substrates lies in the fact that their results can be directly observed on the laboratory bench and permanently preserved through photography, eliminating the need for a darkroom or professional imaging system. The chromogenic substrates provided by KPL are optimized for the detection of HRP (Horseradish Peroxidase) and AP (Alkaline Phosphatase), and are available in multiple sensitivity levels to ensure high stability and batch-to-batch consistency. TMB 1 Membrane Peroxidase Substrate Product Series TMB Membrane Peroxidase Substrate is a high-sensitivity chromogenic substrate, specifically designed for the detection of HRP (Horseradish Peroxidase)-labeled molecules. It can form clear dark blue precipitates at HRP-labeled sites. This substrate is available in two forms: TMB 1-Component Membrane Peroxidase Substrate: A ready-to-use single-component form that is convenient to use with no additional preparation required. TMB Membrane Peroxidase Substrate System (3-C): A three-component system. By omitting the enhancer, it can be adjusted to a soluble substrate system suitable for ELISA, enabling multi-purpose use of one product. Both forms offer excellent sensitivity and application flexibility, and are suitable for various detection methods such as Western Blot, Dot Blot, and ELISA. Their high sensitivity, ease of operation, versatility, and high stability make them one of the most sensitive chromogenic solutions for membrane detection, ensuring the reliability and reproducibility of experimental results. Product Name Item Number Specification Reactive Enzyme / Catalytic Enzyme Signal Type Sensitivity TMB 1-Component Membrane Peroxidase Substrate 5420-0029 5420-0027 5420-0028 100 mL 200 mL 1000 mL HRP 顯色 10-12 TMB Membrane Peroxidase Substrate System（3-C） 5420-0025 440 mL HRP 顯色 10-12 BCIP/NBT Phosphatase Substrate Series BCIP/NBT phosphatase substrates are high-efficiency chromogenic detection substrates specifically designed for use with alkaline phosphatase (AP)-conjugated conjugates. They produce clear purple precipitated bands while maintaining extremely low background staining. This substrate is available in two forms: the classic three-component system, BCIP/NBT Phosphatase Substrate System (3-C), and the more convenient single-component BCIP/NBT 1-Component Phosphatase Substrate ready-to-use solution. Both forms are suitable for Western blotting and ELISpot assays, meeting different experimental needs and ensuring high sensitivity and reliability of detection results. Product Name Item Number Specification Reactive Enzyme / Catalytic Enzyme Signal Type Sensitivity BCIP/NBT 1-Component Phosphatase Substrate 5420-0038 5420-0033 5420-0036 5420-0037 100 mL 6x100 mL 1000 mL 5000 mL AP 顯色 10-12-10-9 BCIP/NBT Phosphatase Substrate System（3-C） 5420-0030 300 mL AP 顯色 10-12-10-9 About the LGC·Seracare·KPL Brands KPL (Kinetic Plasmonics Laboratories) is a company with a profound history and an outstanding reputation in the field of biological sciences. As one of the world’s early biotech companies to commercialize affinity-purified secondary antibodies, KPL has laid a solid foundation for its development by virtue of its pioneering position in this field. In addition, KPL is also one of the world’s leading manufacturers of secondary antibodies and chromogenic substrate systems, boasting nearly 40 years of experience in product R&D, as well as a wealth of professional knowledge and technical capabilities accumulated over the years. The company has obtained ISO 13485 quality management system certification, which ensures that all its products have minimal batch-to-batch variation and reliable quality. Characterized by high purity, high sensitivity, and an excellent signal-to-noise ratio (strong signal with low background), KPL’s products are highly trusted by researchers and diagnostic enterprises. In 2013, KPL was acquired by SeraCare, a supplier of in vitro diagnostic (IVD) reagents, further consolidating its leading position in the industry. Founded in 1984, SeraCare is a major partner for global IVD manufacturers and clinical laboratories, and became part of the LGC Group in 2018. As the exclusive distributor of the LGC·SeraCare·KPL brand in China, Beijing XMJ Biotechnology Co., Ltd. is committed to promoting KPL’s high-quality products in the Chinese market and providing domestic researchers with premium products and professional services. more>

Uncovering Virusys Human Herpes Virus Antibodies: A Research Tool from Laboratory to Clinic

When approximately 90% of adults worldwide carry at least one type of herpes virus (such as HSV, EBV, and CMV), tools for the accurate detection and research of these "latent pathogens" become the core competitiveness in virology research. As a brand under the LGC Group focusing on virology reagents, Virusys has established itself as a reliable partner for researchers unraveling the mysteries of herpes viruses, backed by its strong capabilities—offering over 70 highly specific monoclonal antibodies and having one-third of its products featured in academic journals.Herpes viruses are a group of enveloped double-stranded DNA viruses belonging to the Herpesviridae family. To date, more than 100 types of herpes viruses have been identified. Herpes viruses associated with human infections are referred to as Human Herpesviruses (HHV). Based on their genetic structure, homology, and related characteristics, human herpes viruses are classified into three subfamilies: Alphaherpesvirinae, Betaherpesvirinae, and Gammaherpesvirinae. Among them, HSV-1, HSV-2, and VZV belong to the Alphaherpesvirinae subfamily; HCMV, HHV-6, and HHV-7 belong to the Betaherpesvirinae subfamily; and EBV and HHV-8 belong to the Gammaherpesvirinae subfamily. These viruses can cause a variety of diseases, ranging from common oral and genital herpes to severe congenital infections and certain types of cancer. Understanding these viruses is conducive to better research on their biological characteristics, pathogenic mechanisms, and the development of corresponding therapeutic methods. Herpes Virus SubfamilyOfficial NamingCommon NameTarget CellTissue TropismAlpha(α)Human Herpesvirus Type 1 (HHV-1)Herpes Simplex Virus Type 1 (HSV-1)Mucosal Epithelial CellsGanglionHuman Herpesvirus Type 2 (HHV-2)Herpes Simplex Virus Type 2 (HSV-2)Mucosal Epithelial CellsGanglionHuman Herpesvirus Type 3 (HHV-3)Varicella-Zoster Virus (VZV)Mucosal Epithelial Cells / T CellsGanglionBeta(β）Human Herpesvirus Type 5 (HHV-5)Human Cytomegalovirus (HCMV)Macrophages / Monocytes / Epithelial CellsWhite Blood Cells / Epithelial CellsHuman Herpesvirus Type 6 (HHV-6)Human Herpesvirus Type 6（HHV-6）T Lymphocytes / Monocytes / MacrophagesT LymphocytesHuman Herpesvirus Type 7(HHV-7)Human Herpesvirus Type 7（HHV-7）T Lymphocytes / Epithelial Cells / FibroblastsT LymphocytesGamma(γ)Human Herpesvirus Type 4 (HHV-4)EB virus（EBV）B Lymphocytes / Mucosal Epithelial CellsB Lymphocytes (abbreviation: B Cells)Human Herpesvirus Type 8 (HHV-8)Kaposi's Sarcoma-Associated Herpesvirus (KSHV)Lymphocyte (plural: Lymphocytes)Lymphocyte (plural: Lymphocytes)1. Herpes Simplex Virus (HSV)Herpes Simplex Virus (HSV) is a major pathogen that widely infects humans, mainly classified into two types: HSV-1 and HSV-2. Its infection can cause a variety of diseases, ranging from oral herpes to severe encephalitis. An in-depth understanding of the HSV life cycle is the foundation for developing effective diagnostic methods and intervention strategies.HSV infection relies on a series of key proteins, among which envelope glycoproteins (such as gD, gB, gC, gE, gG, and gH/gL) are core targets. Specifically, gD is responsible for initiating binding to host receptors and serves as the primary target of neutralizing antibodies; while gB, due to its high conservation, has become a preferred target for the development of vaccines and therapeutic antibodies. Immediate-early proteins (such as ICP0 and ICP4) act as "switches" for viral gene expression—their presence marks the onset of viral infection and represents important potential targets for antiviral drug screening.Targeting these core molecules, Virusys offers a range of validated, high-quality HSV antibodies. Whether for glycoprotein research aimed at uncovering viral entry mechanisms, or for the detection of immediate-early proteins during the early stage of viral replication, our products provide accurate recognition, flexible application, and reliable data support. We are committed to accelerating your research in the fields of vaccine development, diagnostic tool creation, and pathogenic mechanism exploration. Item NumberEnglish NameSpecificationVS-HA056-1HSV gB Monoclonal Antibody 1 mgVS-P1122HSV gB Monoclonal Antibody (H126) 500 µgVS-P1123HSV gB Monoclonal Antibody (H1379) 500 µgVS-P1105HSV gB Monoclonal Antibody (H1817) 500 µgVS-P1130HSV gB-1 Monoclonal Antibody (H1435) 500 µgVS-P1132HSV gB-1 Monoclonal Antibody (H1815) 500 µgVS-P1133HSV gB-1 Monoclonal Antibody (H233) 500 µgVS-P1104HSV gC-1 Monoclonal Antibody (H633) 500 µgVS-HA025-100HSV gD Monoclonal Antibody100 µgVS-P1103HSV gD Monoclonal Antibody (H170) 500µg500 µgVS-P1108HSV gE-1 Monoclonal Antibody (H600) 500 µgVS-P1109HSV gE-2 Monoclonal Antibody (H222) 500 µgVS-P1107HSV gG-1 Monoclonal Antibody (H1379) 500 µgVS-P1106HSV gG-2 Purified Monoclonal Antibody 500 µgVS-P1119HSV ICP27 Monoclonal Antibody (H1142) 500 µgVS-P1113HSV ICP27 Monoclonal Antibody (H1113) 500 µgVS-P1101HSV ICP4 Monoclonal Antibody (H943) 500 µgVS-P1120HSV ICP6 Monoclonal Antibody (H121) 500 µgVS-P1115HSV ICP8 Monoclonal Antibody (H793) 500 µgVS-H1A022-100HSV-1 gC Monoclonal Antibody 100 µgVS-H1A054-100HSV-1 gE Monoclonal Antibody100 µgVS-H1A020-100HSV-1 gG Monoclonal Antibody 100 µgVS-H1A258-100HSV-1 gH Monoclonal Antibody 100 µgVS-H1A259-100HSV-1 gL Monoclonal Antibody 100 µgVS-H1A027-100HSV-1 ICP0 Monoclonal Antibody 100 µgVS-H1A021-100HSV-1 ICP4 Monoclonal Antibody 100 µgVS-H2A260-100HSV-2 gC Monoclonal Antibody 100 µgVS-H2A055-100HSV-2 gE Monoclonal Antibody 100 µgVS-H2A023-100HSV-2 gG Monoclonal Antibody 100 µgVS-H2A261-100HSV-2 gH Monoclonal Antibody 100 µgVS-H2A269-100HSV-2 gH/gL Monoclonal Antibody100 µgVS-H2A262-100HSV-2 gL Monoclonal Antibody 100 µgVS-H2A024-100HSV-2 ICP8 Monoclonal Antibody 100 µg 2. Varicella-Zoster Virus (VZV)Varicella-Zoster Virus (VZV) is a highly contagious human herpesvirus that can cause primary infection (varicella, commonly known as chickenpox) and reactivation of latent virus in the body (herpes zoster, commonly known as shingles).Virusys’ VZV antibody library focuses on the glycoprotein family essential for viral replication. Glycoprotein E (gE) is the most abundant envelope glycoprotein of VZV, which dominates viral cell-to-cell spread and immune evasion; while glycoprotein B (gB), as a key fusion protein, plays an irreplaceable role in the initial steps of viral entry into host cells.Targeting key molecules such as gE and gB, Virusys provides validated VZV antibodies with high specificity. These antibodies can not only accurately localize the site of latent viral infection through techniques like immunofluorescence but also have become powerful visualization tools for uncovering the "latency-reactivation" switching mechanism of the virus and advancing related diagnostics and research. Item NumberEnglish NameSpecificationVS-VA032-100VZV gB Monoclonal Antibody 100 µgVS-VA315-100VZV gE Monoclonal Antibody100 µgVS-VA034-100VZV gI Monoclonal Antibody 100 µgVS-VA036-100VZV IE62 Monoclonal Antibody100 µg 3. Cytomegalovirus (CMV)Cytomegalovirus (CMV) is a herpesvirus that is highly prevalent in the human population but typically asymptomatic. However, it can cause severe diseases in immunocompromised individuals and newborns with congenital infections.Virusys’ CMV antibodies target different stages of the viral life cycle (e.g., latency, immediate-early, late stages). The late antigen pp65 (UL83) is a key marker of active viral replication, and its expression in the late stage of infection serves as a core observation window for clinical diagnosis and research.Targeting this critical molecule, Virusys provides high-quality CMV antibodies, represented by the CH12 antibody. As the "gold standard" for clinical antigenemia testing, this antibody can accurately localize viral assembly sites. Thanks to its unique unconjugated design, Virusys’ antibody has been widely cited in numerous top-tier scientific journals worldwide. Whether for research on congenital CMV infections or exploration of CMV replication mechanisms, it is a reliable tool you can trust. Item NumberEnglish NameSpecificationVS-CA005-100CMV gB Monoclonal Antibody 100 µgVS-P1212CMV gB Monoclonal Antibody (CH177) 500 µgVS-P1216CMV gB Monoclonal Antibody (CH253) 500 µgVS-P1201CMV gB Monoclonal Antibody (CH28) - Non-neutralizing500 µgVS-CA010-100CMV ICP22 Monoclonal Antibody 100 µgVS-P1210CMV ICP22 Monoclonal Antibody (CH41) 500 µgVS-CA006-100CMV ICP36 Monoclonal Antibody 100 µgVS-P1202-1CMV ICP36 Monoclonal Antibody (CH13) 500 µgVS-P1202-2CMV ICP36 Monoclonal Antibody (CH16) 500 µgVS-P1209CMV ICP8 (UL57) Monoclonal Antibody (CH167) 500 µgVS-P1203CMV IE1 Monoclonal Antibody (CH443) 500 µgVS-P1215CMV IE1/2 Monoclonal Antibody (CH160) 500 µgVS-CA004-100CMV pp28 Monoclonal Antibody 100 µgVS-P1207CMV pp28 Monoclonal Antibody (CH19) 500 µgVS-CA003-100CMV pp65 Monoclonal Antibody 100 µgVS-P1205CMV UL83 Monoclonal Antibody (CH12)500 µgVS-CA144-500CMV UL84 Monoclonal Antibody 500 µg 4. Epstein-Barr Virus (EBV)Epstein-Barr Virus (EBV) is a human herpesvirus with extremely widespread infection. It is not only a common cause of infectious mononucleosis but also closely associated with various malignant tumors such as multiple types of lymphoma and nasopharyngeal carcinoma.During the pathogenic process of EBV, the immediate-early protein Ea-D serves as the "master switch" of the viral lytic cycle and is key to uncovering viral reactivation and exploring pathogenic mechanisms.With high specificity, Virusys-brand EBV antibodies can accurately recognize Ea-D, making them reliable tools for your research on EBV infection diagnosis, viral reactivation mechanisms, and oncogenic mechanisms. Item NumberEnglish NameSpecificationVS-EA069-100EBV Ea-D Monoclonal Antibody 100 µgVS-EA068-100EBV gp110 Monoclonal Antibody 100 µg 5. Virusys Polyclonal Antibody IgYVirusys polyclonal antibody IgY is produced by immunizing animals with specific antigens, followed by serum collection, purification via Protein G, and purity verification using SDS-PAGE—its purity reaches over 90%. This antibody exhibits high sensitivity and broad reactivity, making it suitable for enzyme-linked immunosorbent assay (ELISA), Western blot, immunohistochemistry (IHC), and other immunological assays.Virusys polyclonal antibodies undergo strict production quality control, which ensures batch-to-batch consistency and high specificity, effectively preventing cross-reactivity. Item NumberEnglish NameSpecificationVS-CA150-1CMV Chicken IgY Ab - 1ml1 mlVS-EA223-1EBV Chicken IgY Antibody – 1 ml 1 mlVS-VA224-1Varicella Zoster Chicken IgY Ab – 1 ml 1 mlVS-VA225-1VZV Glycoproteins Chicken IgY Ab – 1 ml 1 mlVS-GA230-1Normal Chicken IgY 1 mL1 ml Why Choose Virusys Viral Antibodies? Comprehensive Target CoverageOur antibodies cover key targets across the full spectrum of human herpesviruses, including common types such as HSV-1/2 and VZV, as well as tumor-associated viruses like EBV and CMV. They enable accurate recognition of various viral antigens, including matrix proteins, DNA-binding proteins, and membrane proteins. Flexible Experimental DesignAll antibodies are provided in an unconjugated format, allowing researchers to freely pair labels and detection systems according to experimental needs. They seamlessly adapt to multiple technical platforms, such as immunofluorescence (IF), Western blot (WB), and enzyme-linked immunosorbent assay (ELISA). Excellent Quality AssuranceLeveraging hybridoma technology and serum purification techniques, combined with strict purification processes, we ensure all antibodies possess high specificity, high affinity, and high purity. Each antibody is validated to be free of active viral residues. Brand Quality CertificationAs a member of the LGC Group, our production complies with the ISO 13485 international quality management system. Product quality undergoes rigorous quality control, and our antibodies have been widely cited and recognized in numerous academic publications. Virusys CorporationVirusys Corporation is a biotechnology company dedicated to virology-related products and services. Founded in 1999 and headquartered in Taneytown, Maryland, USA, it specializes in virology-related products and services, and is renowned for its high-quality biological research reagents, professional monoclonal antibody technology, a wide range of viral detection products, and flexible custom services.The company’s products are widely used in fields such as vaccine development, diagnostic reagent development, drug screening, and basic research, providing important tools for the prevention and control of infectious diseases and biomedical research. All Virusys antibody products undergo strict quality control and comply with the ISO 13485 quality management system standards to ensure batch-to-batch consistency and high specificity.In October 2023, Virusys Corporation was acquired by the LGC Group and became part of LGC’s Clinical Diagnostics business. This acquisition has strengthened LGC’s product portfolio in the field of infectious diseases and further enhanced Virusys’ market position and influence in the virology sector. As the China-based agent for the Seracare (Virusys) brand, Beijing XMJ Biotechnology Co., Ltd. is committed to promoting Virusys viral antibody products in China and providing high-quality products and professional services to a large number of domestic researchers. If you are interested in the products, please feel free to send email to info@xmjsci.com or visit the official website: m.xh867.cn for more information. more>

IDKmonitor? Ustekinumab Drug Concentration Detection Kit

Ustekinumab is a human therapeutic monoclonal antibody targeting p40, the common subunit of interleukin-12 and interleukin-23 (IL-12/23). These two cytokines play a fundamental role in immune-mediated inflammatory diseases such as Crohn’s disease, psoriasis, and multiple sclerosis. The Ustekinumab Drug Concentration Detection Kit is used to measure the concentration of free ustekinumab (e.g., Stelara&reg;), providing attending physicians with the opportunity for early monitoring and treatment optimization.Immundiagnostik (IDK) GmbH (Germany), founded in 1986, is a global supplier of diagnostic reagents and services. It offers a comprehensive range of in vitro diagnostic (IVD) kits for clinical disease research, covering areas including cardiovascular diseases, musculoskeletal disorders, oxidative stress, and oncology. These kits are applicable for detecting samples from different matrices (e.g., blood, urine, feces, tissue culture supernatants, etc.) and across various species (e.g., humans, mice, rats, etc.).The IDKmonitor&reg; Ustekinumab Drug Concentration Detection Kit from Immundiagnostik (IDK) is designed for the in vitro quantitative determination of free therapeutic ustekinumab antibodies (e.g., Stelara&reg;) in human serum and EDTA plasma. For Research Use Only. Item NumberK 9660Specification96TestSample TypeSerum, EDTA PlasmaSample size10µlIncubation Time1h/1h/10-20minStorage ConditionsThis product remains stable until the indicated expiration date when stored at 2–8°C.Applicable InstrumentsIt is suitable for all fully automatic and semi-automatic microplate readers with wavelengths of 450 nm and 620 nm. More Monoclonal Antibody Drug Concentration Detection:Product NameItem NumberIDKmonitor&reg; Free Anti-Infliximab Antibody Detection KitK9650IDKmonitor&reg; Total Anti-Infliximab Antibody Detection KitK9654IDKmonitor&reg; Infliximab Drug Concentration Detection KitK9655IDKmonitor&reg; Free Anti-Adalimumab Antibody Detection KitK9652IDKmonitor&reg; Total Anti-Adalimumab Antibody Detection KitK9651IDKmonitor&reg; Adalimumab Drug Concentration Detection KitK9657IDKmonitor&reg; Free Anti-Etanercept Antibody Detection KitK9653IDKmonitor&reg; Etanercept Drug Concentration Detection KitK9646IDKmonitor&reg; Free Anti-Golimumab Antibody Detection KitK9649IDKmonitor&reg; Golimumab Drug Concentration Detection KitK9656IDKmonitor&reg; Free Anti-Vedolizumab Antibody Detection KitK9648IDKmonitor&reg; Vedolizumab Drug Concentration Detection KitK9658IDKmonitor&reg; Free Anti-Ustekinumab Antibody Detection KitK9666IDKmonitor&reg; Total Anti-Ustekinumab Antibody Detection KitK9667IDKmonitor&reg; Ustekinumab Drug Concentration Detection KitK9660 Beijing XMJ Technology Co., Ltd. is the China distributor of Immundiagnostik (IDK), providing customers with comprehensive technical support and after-sales service. For more information, please feel free to send email to info@xmjsci.com or visit the official website at m.xh867.cn. more>

About Us

Introduction

Beijing XMJ Technology Co., Ltd., founded in 2005, is a supplier of reagent raw materials and auxiliary materials focusing on the fields of life sciences, biopharmaceuticals and related areas. It is committed to providing comprehensive product solutions and technical support services for scientific research institutions more>

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